Drug Metabolism and Pharmacokinetics

Harlan Laboratiories performs all metabolism studies necessary to characterise the absorption, tissue distribution and depletion, metabolism and elimination profiles in vivo and to interpret toxicological findings.

• ¹⁴C, ³H and ¹²⁵I-radiolabelled materials
• All relevant species
• All administration routes, single / multiple dosing

A set of tests, mainly in vitro, is available to study specific reaction pathways and to predict drug-drug interactions:

• CYP interaction, inhibition and induction
• Metabolic stability with liver S9 preparation
• Phase II reactions, i.e. conjugation
• Protein binding
• Dermal penetration

Analytical techniques to characterise and identify metabolites:

• HPLC, GC, CE, TLC
• LC-MS/MS and GC-MS
• Whole body autoradiography (QWBA)
• Real-time (quantitative) PCR
• Access to NMR