Inhalation Toxicology

 

Literature

Harlan Laboratories offers a complete range of toxicology studies to support the world-wide registration of inhaled pharmaceuticals or chemicals

  • Acute toxicity in rodent
  • Subacute to subchronic toxicity in rodents and non-rodents
  • Chronic toxicity in rodents and non-rodents
  • Reproduction and teratogenicity in rats and rabbits
  • Carcinogenicity
  • ADME and toxicokinetic studies can be performed in rodents and dogs using radiolabelled test items 
  • Safety pharmacology studies can be performed according to ICH S7A guidelines. 

Harlan Laboratories is one of the few facilities that combines inhalation dosing expertise with general and reproductive toxicology and pharmacology. Various aerosol generation methods are employed at Harlan Laboratories, the choice of test system being dependent on the physical characteristics of the test item. Systems include the use of double brush-feed and piston-feed generators, with or without a jet-mill microniser, for dry powders; nebulisers for liquids and specialised equipment for the administration of gases and test compounds administered by metered dose inhaler (MDI).

Studies are performed in purpose-built laboratories with on-line facilities for data capture from animals and exposure systems. 

Specialised exposure methods are available for rodents, rabbits and dogs. In rodents and rabbits the use of a positive-feed, flow past, nose-only system ensures that all animals receive a consistent concentration of ’fresh’ aerosol throughout the exposure. For dogs, a muzzle-only exposure system based on similar principles is routinely used. Each inhalation system can be adapted for use with dry powders, liquids or gases (i.e. CFC-replacement propellants) taking into consideration the biological, physical and chemical properties of each test item. Inhalation studies are perfomed in rodents for up to two years and in dogs for up to one year.

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