Genetic Testing Services >> Inbred Strain Verification >> Strain Microsatellite Profiling

The mouse and rat microsatellite marker panels used in the Harlan GenCheck™ program are derived from numerous data resources. Depending on the specific program, more than 20 markers are utilized to study strain dependant polymorphic markers of identity. All markers have been optimized, validated and referenced against a large number of inbred strains. All PCR is performed using liquid handling automation to ensure accurate and efficient data generation. Following amplification, Lab-on-a-Chip technology from Caliper (AMS90) is employed to detect and size all DNA products. This provides a sensitivity of +/- 2 base pairs on sizing the band of interest and also ensures that even the lowest copy number samples are detected. Finally, all data is analyzed and presented in a user friendly proprietary Harlan GenScreen™ database that is accessible through the World Wide Web using an independent and secure login for all Harlan GenCheck™ program participants. Data analysis for each project employs the use of novel algorithms that weigh within screen, across screen and reference database information to make accurate, reproducible and informed determinations.