Alternative & In vitro toxicity

Harlan Laboratories is committed to the principle of the 3Rs of Replacement, Reduction, and Refinement. Harlan Laboratories is proactive in the development and use of in vitro and ex vivo methods, partnering researchers and clients in pre-validation and formal validation studies.
In silico safety modeling (structure activity relationship by computerized models)

Acute toxicity

• Assessment of starting dose for acute toxicity
• Target organ toxicity with primary cells and cell lines from various organs
• Phototoxicity using Balb/3T3 cells and neutral red uptake

Eye irritancy

• Bovine Corneal Opacity and Permeability test (BCOP)
• Hens Egg Chorioallantoic Membrane test (HET-CAM)
• Reconstructed human corneal epithelial models
• Rabbit Enucleated Eye Tests (REET)
• Red Blood Cell test (RBC)

• Transcutaneous Electrical Resistance assay (TER)
• Corrositex™ membrane barrier test

• Reconstructed human skin equivalents

• Dermal penetration using flow-through diffusion cells and human pig isolated skin systems
• Intestinal uptake using CaCo-2 absorption models
• Blood brain barrier system using pig brain microvascular endothelial cells

In vitro  metabolism

• Metabolic stability and metabolic profiling
• Interspecies comparative metabolism
• Selective human cytochrome P450 metabolism
• Human cytochrome P450 inhibition and induction
• MDE (p-glycoprotein) interaction assay

• Embryonic mouse stem cell test

• Cell transformation assays using SHE and Balb/c 3T3 cells