Overview
Harlan Laboratories offers antibody conjugation services and
antigen conjugation services. Choosing the best conjugate for your
application is not always easy. We can help you decide on the most
suitable conjugate for your applications.
Biotin
- Biotin is the conjugate of choice if the antibody is going to
be used in a variety of applications. Biotin is a small molecule
that has little effect on an antibody’s immuno reactivity. Its
small size makes biotin an excellent tag for your antibody in most
techniques. Biotin binds tightly to avidin or streptavidin, which
are available enzyme conjugated, making your biotinylated antibody
useful in most immunological procedures. The disadvantage of a
biotinylated antibody over an enzyme or fluorescent conjugated
antibody is that there is an additional step involved in your
assay.
HRP and Alkaline
Phosphatase
- Enzyme conjugates give a highly selective and sensitive
immuno-reagent. By directly labelling your primary antibody you
eliminate steps and washes.
- Horseradish Peroxidase is a stable enzyme suitable for ELISA,
Western Blot and Immunocytochemistry. HRP has low endogenous
activity and a high turnover rate, producing its coloured end
product in a short time period.
- Alkaline Phosphatase is often used when high background is
experienced with HRP interacting with endogenous peroxidases.
Alkaline Phosphatase allows linear reaction rates improving
sensitivity by reacting over longer time periods.
Fluorescent
Markers
- Fluorescent probes are used in multiple probe assays,
immunofluorescence microscopy and flow cytometry. A minimum
quantity of 2mgs of antibody is required for all
conjugations.
Contact us at antibodies@harlan.com for
pricing and additional information.