Overview
If you require a peptide (Amino Acid Sequence) to be
synthesized, you need to provide the desired sequence so we can
produce this to a purity of 85% or 95%. In case the peptide has a
long sequence e.g.: 269mer with certain sections of interest we can
look into this and come up with the most suitable Antigenic Peptide
Sequences and once we have decided which would be more suitable for
your needs the synthesis can begin. It is our standard procedure to
synthesize 10mg of Peptide, 5mg of this is conjugated to KLH and
5mg left over for any further testing you may require. This is
shipped to you at the end of the project if not required
beforehand.
Synthesis
- Peptide synthesis is performed using an insoluble resin support
with a linker attached. This can then be cleaved by TFA () to ensure that the amino
acids couple in the correct orientation the various reactive groups
of inappropriate reactions are protected. These are simultaneously
removed when the peptide is cleaved from the solid support by the
trifluoroacetic acid.
Purification
- Additional chemicals, termed scavengers are dissolved in the
TFA. These absorb the released reactive species which could affect
the purity of the peptide. The peptide may also contain truncated
and deletion sequences which may interfere with activity of the
peptide. Both truncated and deletion sequences and scavengers can
be removed using purification by reverse-phase hplc. For antibody
production purities of greater than 80% are advised.
Analysis
- All peptides are analysed for purity by hplc and mass
spectroscopy. The hplc trace is supplied with each peptide, and
amino acid analysis can be provided on request.
Presentation and
Storage
- Synthesised purified peptides are supplied as lyophilized
powders. Peptides should be stored in a sealed glass vial protected
from light at –20Cº with a desiccant. Avoid repeated freeze/thaws.
Harlan Laboratories reserves the right to review all peptides
before synthesis. If a peptide is determined to require special
procedures, a separate quotation will be issued before synthesis
begins. For conjugation of your peptide to KLH or other carrier
molecules, we refer to the conjugation section.
Peptide
Modifications
- If you require modification of your peptide such as cyclisation
or phosphorylation please contact us for an individual quote.
Harlan offers peptide synthesis using continuous flow Fmoc
(9-fluorenylmethyloxycarbonyl) chemistry.
Contact us at antibodies@harlan.com for
pricing and additional information.