Protocols

 

Information and Research Data
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European Antibody Production Services Summary Brochure
Antibody Services Production Questionnaire
COSHH Assessment for Antibody Services
Antigen Shipping and Receipt Form
Ethical Review Questionnaire

Harlan Laboratories has established antibody production protocols for polyclonal production, alternatively we can use protocols modified to our customers' requirement. Tell us the number of immunizations and bleeds you require, and we will incorporate this into a format for your approval. In order to satisfy regulations, a brief justification of the project to be undertaken is required before the antibody production protocol can commence. All antigens submitted for polyclonal antibody production must be accompanied by a statement (such as e.g. COSHH) detailing all risks associated with the handling of the antigen. A protocol may be extended beyond its original finish date. We ask the end-user to confirm by fax/e-mail 48 hrs in advance of termination.

For providing the optimal service and communication in providing you a high quality end product and to fulfill legal requirements, we ask you to fill out some information sheets, which include:

Following are some of our typical immunization protocols. As previously mentioned these antibody production protocols can be subject to any change as determined by the customer.

Rabbit Immunization Protocol 
DayAction
0Pre Bleed + Immunisation
14Boost 1
28Boost 2
35Test Bleed 1
42Boost 3
49Test Bleed 2
56Boost 4
63Test Bleed 3
70Boost 5
77Terminal Bleed

 

Goat/Sheep Immunization Protocol 
DayAction
0Pre Bleed + Immunisation
21Boost 1
35Boost 2
42Test Bleed 1
56Boost 3
63Test Bleed 2
70Boost 4
77Test Bleed 3
84Boost 5
98Terminal Bleed

 

Chicken Immunization Protocol 
DayAction
0Pre Bleed + Immunisation
14Boost 1
28Boost 2
35Test Bleed 1
42Egg collection up to 10 eggs
77Terminal Bleed

 

Mouse/Rat Immunization Protocol 
DayAction
0Pre Bleed + Immunisation
7Boost 1
14Boost 2
21Test Bleed 1
28Boost 3
35Terminal Bleed

These standard packages have been designed and optimized to work with most antigens. Optional boosts and bleeds can be performed if required. Termination of the project will not be carried out without written approval. Any delays on termination will be charged at list price. 

Contact us at antibodies@harlan.com for pricing and additional information.



 

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