Harlan Laboratories has established antibody
production protocols for polyclonal production, alternatively we
can use protocols modified to our customers' requirement. Tell us
the number of immunizations and bleeds you require, and we will
incorporate this into a format for your approval. In order to
satisfy regulations, a brief justification of the project to be
undertaken is required before the antibody production protocol can
commence. All antigens submitted for polyclonal antibody production
must be accompanied by a statement (such as e.g. COSHH) detailing
all risks associated with the handling of the antigen. A protocol
may be extended beyond its original finish date. We ask the
end-user to confirm by fax/e-mail 48 hrs in advance of
termination.
For providing the optimal service and communication
in providing you a high quality end product and to fulfill legal
requirements, we ask you to fill out some information sheets, which
include:
Following are some of our typical immunization
protocols. As previously mentioned these antibody production
protocols can be subject to any change as determined by the
customer.
| Rabbit Immunization Protocol | |
| Day | Action |
| 0 | Pre Bleed + Immunisation |
| 14 | Boost 1 |
| 28 | Boost 2 |
| 35 | Test Bleed 1 |
| 42 | Boost 3 |
| 49 | Test Bleed 2 |
| 56 | Boost 4 |
| 63 | Test Bleed 3 |
| 70 | Boost 5 |
| 77 | Terminal Bleed |
| Goat/Sheep Immunization Protocol | |
| Day | Action |
| 0 | Pre Bleed + Immunisation |
| 21 | Boost 1 |
| 35 | Boost 2 |
| 42 | Test Bleed 1 |
| 56 | Boost 3 |
| 63 | Test Bleed 2 |
| 70 | Boost 4 |
| 77 | Test Bleed 3 |
| 84 | Boost 5 |
| 98 | Terminal Bleed |
| Chicken Immunization Protocol | |
| Day | Action |
| 0 | Pre Bleed + Immunisation |
| 14 | Boost 1 |
| 28 | Boost 2 |
| 35 | Test Bleed 1 |
| 42 | Egg collection up to 10 eggs |
| 77 | Terminal Bleed |
| Mouse/Rat Immunization Protocol | |
| Day | Action |
| 0 | Pre Bleed + Immunisation |
| 7 | Boost 1 |
| 14 | Boost 2 |
| 21 | Test Bleed 1 |
| 28 | Boost 3 |
| 35 | Terminal Bleed |
These standard packages have been designed and optimized to work
with most antigens. Optional boosts and bleeds can be performed if
required. Termination of the project will not be carried out
without written approval. Any delays on termination will be charged
at list price.
Contact us at antibodies@harlan.com for
pricing and additional information.