Overview
- The technique used to process the sera ensures high complement
activity and is in line with the recommendations of the European
Health Committee. The complement is screened by a standard
haemolytic titration assay against the British Reference Reagent.
Endogenous non-specific cytotoxicity in the absence of antibody has
been quantified. Because of the variation in complement fixation
tests and assays, Harlan can supply a free of charge sample for
testing against reserved batches. Complement can be collected from
Guinea pigs, Rabbits and baby rabbits and is available frozen or
lyophilised. Complement products include antibody complement
and serum complement.
Guinea Pig Complement
- Harlan Laboratories' Guinea Pig complement is collected
from pooled sera of healthy animals that have been raised under
closely defined conditions, therefore minimizing lot-to-lot
variation.
Rabbit Complement
- All rabbit complement is collected from healthy rabbits and the
technique used to process the sera ensures high complement
activity. The complement is tested by appropriate assays to ensure
high activity and low cytotoxicity.
Baby Rabbit Complement
- Normal rabbit serum is collected and pooled from young rabbits
21-28 days old. The complement is prepared in line with standard
lymphocytotoxicity techniques.
Rabbit Complement for use with Mouse
Lymphocytes
- Rabbit serum is normally cytotoxic to mouse lymphocytes,
particularly thymocytes. The cytotoxicity in this complement has
been significantly reduced by absorption with mouse tissues and
agarose.
Harlan uses a number of selected major couriers to ship all
products direct to your facility. Please discuss your
specifications with us, so we deliver the right product to you,
meeting your research needs.
Contact us at biologicals@harlan.com for
pricing and availability.