Services and
Offerings
Harlan Bioproducts for Science, Inc. (HBPS) can purify your
monoclonal and polyclonal antibodies utilizing a wide array of
protein purification methods; from research (mg) to scale-up
(grams) quantities. We also offer low endotoxin production for
in-vivo use. We are happy to discuss your antibody
purification options. Our goal is to find or develop the antibody
purification procedure which provides the best balance between
yield, purity, and cost. This process is initiated by HBPS sending
you a questionnaire to determine your requirements. Available
Antibody Purification services and methods
include:
- Protein G affinity chromatography
- Protein A affinity chromatography
- Ion-exchange chromatography (anion and cation)
- Hydrophobic interaction chromatography
- Antigen affinity
- Size exclusion chromatography
- Ammonium sulfate precipitation
- HPLC purification
1) Ammonium Sulphate Precipitation
This method is useful for concentration and partial purification
of antibodies from most sources and all species. Although, on its
own the yields of antibody are impure, it is the method of choice
when combined with ion exchange for large volumes of anti-sera. It
is not recommended for purification of tissue culture
supernatant.
2) Protein A
This process binds the Fc region of major sub-classes of mouse
and rat IgG to varying degrees. The results with protein A are
usually high purity and high yields. It is useful for large-scale
purifications of tissue culture
supernatants.
3) Protein G
Protein G can be used for purifying antibody from most sources.
Like protein A, the results are usually of high purity and high
yield. It is applicable to a wider range of immunoglobulins than
protein A, but elution procedures can be much harsher.
4) Ion Exchange Chromatography
This method is used for all sources of antibodies. It is
applicable to all lgG isotypes. It is usually the method of choice
when purifying antibodies from ascites, as the monoclonal can be
resolved from the host immunoglobulins.
5) Ig Affinity
This method can be used to isolate mouse IgM and IgG3, and for
isolating monoclonal antibodies from supernatants which contain
high levels of serum Ig.
6) Antigen Affinity
This is the method of choice when specific antibody is required
from polyclonal anti-sera. It requires pure antigen for the best
results and can sometimes result in inactive antibody.
7) Size Exclusion
This method is appropriate for IgM antibodies from all sources.
It is used when IgM antibodies need to be separated from polyclonal
anti-sera. It is not recommended as a single stage.
Contact Information
By using HBPS purification in conjunction with our custom
monoclonal antibody development and production services, you obtain
complete service quickly and efficiently.
Contact our Customer Services Center at 800-972-4362 or email us
at hbps@harlan.com to receive
a
Purification Information Sheet, pricing, or for more
information about our capabilities.