Mouse Antibody Production (MAP) Testing

Services and Offerings

• Mouse Antibody Production (MAP) testing requires about 25 x 10⁶ cells. Three groups of mice will be inoculated: 1) cell suspension undiluted, 2) cell suspension diluted 1:5, and 3) controls which are sham inoculated with PBS.  Each mouse will be inoculated by the intraperitoneal and intranasal routes.  The mice are examined once a day for 28 days for signs of disease or mortality.
• Twenty-eight days after inoculation, the test animals and the control animals will be bled.  The test sera and control sera are tested for antibody. (Note that PCR testing is also available upon request.)
• The standard panel includes testing the following agents:
  • MHV
  • Polyoma
  • Sendai
  • MVM
  • Parvovirus NS-1
  • PVM
  • LCM
  • Hantaan
  • REO 3
  • GDVII (TMEV)
  • K Virus
  • MCMV
  • Ectromelia
  • EDIM (Rotavirus) 
  • MPV
  • LDEV
  • MTV
  • MAD1
  • (M. Pulmonis [culture]/serology) 
• The panel can be expanded, as an option, to include the following:
  • MAD 2
  • ECUN
  • MNV
• Expanded LCM testing can also be provided as an option.

Contact Information

• To receive more information about our capabilities or pricing, contact our Customer Services Center at  800-972-4362 or email us at hbps@harlan.com. Standard timeline is 6 weeks.