Harlan Bioproducts for Science, Inc. (HBPS) has over 20 years of
polylconal antiserum production experience from a wide array of
antigens. They include purified proteins, fusion proteins,
synthetic peptides, bacteria, fungi, and others. All of our
production is done in our USDA-licensed facility (32-R-0035).
We offer production from, rabbit, goat, sheep, chicken, rat
mouse and guinea pig using our standard protocols or we can
follow your specific protocol. An example of our
standard protocols are as follows:
Rabbit Antibody Production: Sample 112-day
Protocol
| Day | Procedure |
| | |
| 0 | Pre-Bleed (avg. 5ml)/Primary Immunization |
| 28 | Boost |
| 42 | Test bleed (avg. 5ml) |
| 56 | Boost |
| 70 | Test bleed (avg. 5ml) |
| 84 | Boost |
| 98 | Production bleed (~25ml) |
| 105 | Production bleed (~25ml) |
| 112 | Final bleed (~60ml) |
This protocol can be extended to include additional
boosts evey 28 days and production bleeds every 14 days.
Goat Antibody Production
Protocol: 1 Goat, 90-Day Protocol
| Day | Procedure |
| | |
| 0 | Pre-bleed/initial inoculation (1mg of Ag) |
| 14 | Boost (500 ug) |
| 21 | Boost (500 ug) |
| 35 | Test bleed (~5-10 ml) |
| 49 | Boost (500 ug) |
| 56 | Test bleed (~5-10 ml) |
| 63 | Production bleed (~1 x 350 ml) |
| 74 | Production bleed (~1 x 350 ml) |
| 88 | Exsanguination/Disposal (1 x 1000 ml) |
Mouse Antibody Production Protocol: 2 Mice,
70-Day Protocol
| Day | Procedure |
| | |
| 0 | Pre-bleed /initial inoculation (50 ug of ag) |
| 28 | Boost (25 ug of ag/boost) |
| 42 | Test bleed |
| 56 | Boost (25 ug of ag/boosts) |
| 70 | Final bleed (~0.3 ml serum/mouse) |
Rat Antibody Production Protocol: 2 Rats,
70-Day Protocol
| Day | Procedure |
| | |
| 0 | Pre-bleed/initial inoculation (50 ug of ag) |
| 28 | Boost (25 ug of ag/boost) |
| 42 | Test bleed |
| 56 | Boost (25 ug of a/boosts) |
| 70 | Final bleed (~3-5 ml serum/rat) |
Guinea Pig Antibody Production Protocol:
2 Guinea Pigs, 90-Day Protocol
| Day | Procedure |
| | |
| 0 | Pre-bleed/initial inoculation (200 ug of ag) |
| 14 | Boost (100 ug of ag/boost) |
| 21 | Boost |
| 35 | Test bleed |
| 49 | Boost |
| 56 | Test bleed |
| 63 | Production bleed (1 ml) |
| 77 | Boost |
| 84 | Production bleed (1 ml) |
| 91 | Exsanguination/Disposal (~5-10 ml) |
Contact Information
- To receive a
Polyclonal Information Sheet, pricing, or for more information
about our capabilities, including custom polyclonal production,
contact our Customer Services Center at 800-972-4362 or email at
hbps@harlan.com. Our technical staff is also available to assist
you in determining the type of polyclonal production system that
best meets your needs.