Harlan Bioproducts for Science, Inc. (HBPS) performs polyclonal antibody production in its USDA-licensed facility using pathogen-free, barrier-raised New Zealand White Rabbits (HsdOkd:NZW) obtained from Harlan. Barrier-raised rabbits often produce antibodies with higher titers and provide greater reproducibility between animals than conventional animals. We can produce polyclonal antibody with a wide variety of antigens, from bacteria, viruses, fungi, fusion proteins, synthetic peptides, and plasmid DNA, as well as a variety of other proteins. We typically need a minimum of 1mg of antigen (1mg/ml) to inoculate each animal.
The average serum yield on a 112-day protocol produced by HBPS is approximately 120ml/rabbit. We can produce polyclonal antibodies in a few to several hundred rabbits per run, using our standard protocol (listed below) or by custom protocol, to meet your specific needs. We also supply polyclonal production from other species, including goat, sheep, horse, chicken, rat, mouse, and guinea pig. To obtain further information, contact our National Customer Services Center at 800-9SCIENCE. HBPS supplies a Polyclonal Antibody Production Information Sheet with each inquiry to assist in meeting customer specifications.
Rabbit Antibody Production: Sample 112-day Protocol
| Rabbit Antibody Production: Sample 112-day Protocol |
| Day | Procedure |
| 0 | Pre-bleed (avg. 5ml)/Primary Immunization |
| 28 | Boost |
| 42 | Test bleed (avg. 5ml) |
| 56 | Boost |
| 70 | Test bleed (avg. 5ml) |
| 84 | Boost |
| 98 | Production bleed (~25ml) |
| 105 | Production bleed (~25ml) |
| 112 | Final bleed (~60ml) |
This protocol can be extended to include additional boosts every 28 days and production bleeds every 14 days.
|
| Service | |
| Animal: New Zealand White (HsdOkd:NZW) | |
| Maintenance | |
| Pre-bleed/Primary Immunization | |
| Immunizations/boosts | |
| Test Bleeds (avg. 5ml) | |
| Optional ELISA | |
| Production Bleed (~ 25ml) | |
| Final Bleed (~60ml) | |
| Service | |
| Animals (2 rabbits) | |
| Maintenance (2 rabbits for 112 days) | |
| Pre-bleed/primary immunization (2 x 1) | |
| Boosts (2x3) | |
| Test bleeds (10ml) (2x10) | |
| Production bleeds (~50ml) (2 x 50) | |
| Final bleed (~60ml) (2 x 60) | |
| | |
Rabbit Antibody Production: Sample 73-day Protocol
| Rabbit Antibody Production: Sample 73-day Protocol |
| Day | Procedure |
| 0 | Pre-bleed/Primary Immunization |
| 21 | Boost |
| 31 | Test bleed/ELISA |
| 42 | Boost |
| 52 | Production bleed |
| 59 | Production bleed |
| 63 | Boost |
| 73 | Final bleed (~60ml) |
| Service | |
| Animal: New Zealand White (HsdOkd:NZW) | |
| Maintenance | |
| Pre-bleed/Primary Immunization | |
| Immunizations/boosts | |
| Test Bleeds (avg. 5ml) | |
| Optional ELISA | |
| Production Bleed (~ 25ml) | |
| Final Bleed (~60ml) | |
| Service | |
| Animals (2 rabbits) | |
| Maintenance (2 rabbits for 73 days) | |
| Pre-bleed/primary immunization (2 x 1) | |
| ELISA (Optional) (2 x 1) | |
| Boosts (2x3) | |
| Test bleeds (2x1) | |
| Production bleeds (2x2) | |
| Final bleed (2x1) | |
| |
|
Miscellaneous Information
- Average yield 120 ml antisera/animal.
- 1 mg antigen/animal; 3 mg/animal if antigen is conjugated.
- Conjugation of antigen to KLH is available at $160/conjugation/antigen using the cross-linking agent EDC and $210/conjugation/antigen using sulfo-SMCC.
- Maintenance price will double on animals that are resting >30 days beyond the last protocol.